The Effect Of Ph On Enzymatic Reaction

Title : Factor touching Enzyme Activity (pH) Objectives 1. To read the following(a) instruction manual carefully. 2. To determine the significant do of different pH levels on enzyme activities. 3. Find out the best pH execute for the reactions of the given(p) enzyme through a plan graph. Variables Independent variables : pH of event in streak supply symbiotic variables : quantify for disappearance of straination of the answer Constant variables : Total mass of solution in distributively turn up tube, volume of amylum in apiece mental testing tube, temperature of the solution. Materials Beakers, test tubes in a rack, dropper, marking pen, pipettes, sucker, global index number paper, stop watch, distilled water system, fresh stiffen solution, unity solution, amylase, 0,1 groyne/L hydrochloric vitriolic (pH=1), 0.1mol/L acetic sultry (pH=3), 0.001mol/L atomic number 11 hydrated oxide (pH=11), 0.1 mol/L sodium hydroxide (pH=13), trumpery rod, incise white tile. Procedures 1. The pH of both acidulated and two grassroots was recorded in the control panel below. 2. 5 test tubes with the solution were set up as shown as in the prorogue below. 3. The contents of each test tubes were mixed and the pH was checked. It was indite down to the right of the world-class in the pH newspaper column if the pH is different from the pH recorded. 4.

All the tubes were find ad the date when the blue colour disappeared in each tube was noted. 5. The reciprocal of each time was calculated and the harbor was inserted in the 1/time column. This was use as the exercise of the enzyme. 6. A graph of activity (1/time) against pH was plotted on a graph paper. Results Test tubes |mL starch |mL iodine |mL amylase |mL distilled water |mL of acid or basic |pH |Time for disappearance of colours, t (s) |1/time (s-1) | | | | | | | |Before |After | | | |1 |3 |3 |3 |1 |0 |7 |6 |cl |0.0067 | |2 |3 |3 |3 |0 |1 mL of 0.1 mol/L HCL |1 |3 |240 |0.0042 | |3 |3 |3 |3 |0 |1 mL of 0.1 mol/L acetic acid |3 |5 |195 |0.0051 | |4 |3 |3 |3 |0 |1 mL of 0.001...If you want to get a full essay, put up it on our website: Ordercustompaper.com

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